Milovanov's method / Carbonate - Phosphate method
MILOVANOV'S METHOD/CARBONATE-PHOSPHATE METHOD
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- It is also called as “carbonate phosphate method”.
- It was developed by Milovanov (USSR) during 1959.
- Composition of this media is as follows
Part A
S.No.
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Components
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Quantity
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1
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Potassium dihydrogen phosphate
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72 mg
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2
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Distilled water
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10 ml
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Part B
S.No.
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Components
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Quantity
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1
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Sodium citrate
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2.025 gm
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2
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Glucose
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0.570 gm
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3
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Sodium bicarbonate
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0.125 gm
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4
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Penicillin G sodium
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60,000 mg
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5
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Dihydro streptomycin sulphate
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0.100 gm
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6
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Sulphanilamide
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0.300 gm
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7
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Distilled water
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90,000 ml
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Mix Part A and Part B in the ratio of 1:9.
Part C
- Add 11 ml of egg yolk to the above solution (AB).
- The antibiotics are added as per the schedule.
- One ml of the diluted semen are sealed off in glass ampoules and stored at 20-25oC.
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Last modified: Monday, 11 June 2012, 11:38 AM