Tris - egg yolk - glycerol diluent / universal diluent

TRIS-EGG YOLK-GLYCEROL DILUENT/UNIVERSAL DILUENT

Composition

S.No. Components Quantity
1 Tris Tris 3.028 gm
2 Citric acid Citric acid 1.675 gm
3 Fructose Fructose 1.250 gm
4 Glycerol Glycerol 7 ml
5 Penicillin G sodiumAntibiotic 1 lac unit
6 DihydrostreptomycinAntibiotic 100 mg
7 Distilled waterDistilled water Make upto 80 ml
8 Egg yolkEgg yolk 20 ml
  • Inclusion of glycerol at room temperature was found as satisfactory as 5⁰C.
  • Tris dilutor allows filling and sealing of poly vinyl straws at room temperature while freezing the semen.

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Last modified: Monday, 4 June 2012, 10:22 AM