Veterinary Andrology and Reproductive Techniques

ARTIFICIAL VAGINA (AV)

AV is sterilized in separate autoclave at 5 psi for 20 minutes.

• Cone from the AV and water from the AV jacket should be removed before washing.
• Cones and AVs are cleaned thoroughly with soft sponge brush under running tap water and then soaked in warm neutral cleanser for about 30 minutes, followed by proper rinsing in warm and clean water and lastly washed three times with double distilled water.
• For sterilization, fully assembled AVs should be autoclaved at 5 psi pressure for 20 minutes.
• During sterilization the AV valve should be kept open.
• Finally, the AVs should be stored in the incubator at around 40-50°C for next few days use.
• To achieve best cleaning effect, AVs should be cleaned immediately after use, preferably by non-spermicidal neutral detergent.