Veterinary Andrology and Reproductive Techniques

COLLECTION TUBE

Washed, dried collection vials are sterilized in hot air oven at 160°C for 1 h or at 180°C for 30 minutes.

• The semen collection tube/vial should be immersed in a neutral deteregnt solution immediately after its use.
• It should be washed thoroughly with running tap water and soaked in warm, non-spermicidal neutral detergent solution for about 30 minutes.
• Using appropriate nylon brush, the collection tube should be cleaned in all the sides and bottom to remove the semen particles and rinsed with running tap water.
• Finally, the collection tube should be rinsed three times with double distilled water and allowed to dry in oven at 60°C.
• The open end of the dried collection tube should be closed with aluminium foil an sterilized in hot air oven at 160°C for 1 h or at 180°C for 30 minutes.