Veterinary Andrology and Reproductive Techniques

MATERIALS REQUIRED

Materials required

• Fresh/frozen semen
• Sugar tube
• Distilled water/HOS media
• Water bath
• Pipette and tips
• Preparation of HOS media

For 75 mosm media

• Sodium citrate - 0.367 gm
• Fructose - 0.675 gm
• Distilled water - 100 ml

The osmololity of water is '0'. This can be used as hypo osmotic media. Otherwise hypo osmotic media (with different osmolalities like 75, 100, 125, 150 & 250 mOsm) can be prepared with sodium citrate and fructose in certain combination.