Veterinary Andrology and Reproductive Techniques

Tris-yolk extender

TRIS-EGG YOLK EXTENDER

• Tris-egg yolk diluent can best be used for cattle abd buffalo bull semen.
• It contains 7% glycerol and 20% egg yolk.

• Davis et al (1963) and Good et al (1966) studied certain organic compounds and found excellent buffering capacity when used as semen extenders.
• Tris (hydroxymethyl amino methane (C4H11NO3) is an organic compound which in combination with glucose or fructose proved an ideal semen dilutor.
• Yassen (1970) reported better preservation of buffalo semen with tris extender.
• Recently, Tris dilutor is being extensively used for bull and buffalo bull semen preservation by almost all the advanced countries.
• Tris dilutor may be prepared as follows.

S.No.	Contents	Quantity
1	Tris	3.028 gm
2	Citric acid	1.675 gm
3	Fructose	1.250 gm
4	Glycerol	7 ml
5	Penicillin G sodium	1 lac unit
6	Dihydrostreptomycin	10 mg
7	Distilled water (make up to)	80 ml

• 20% egg yolk is added and mixed properly.
• This dilutor was found to be superior for both Zebu and buffalo bull semen.
• The preservability and the fertility were found to be better those other dilutors.

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