Other diluents used in ultra low temperature

OTHER DILUENTS USED IN ULTRA LOW TEMPERATURE

(Sodium citrate extender, Milk extender and Lactose egg yolk extender)

Sodium citrate extender

  • Egg yolk citrate is prepared as per the procedure given in refrigerated storage of semen except sulphanilamide - because sulphanilamide crystallizes during freezing and hence it is not used in dilutors used for deep freezing).
      • Sodium citrate dihydrate - 2.9 gm
      • Penicillin G sodium - 1 lac unit
      • Dihydrostreptomycin sulphate - 100 mg
      • Distilled water - make up to 100 ml
      • To the above solution 20% egg yolk and 10% glycerol.

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Milk extender

  • The preparation is explained as milk diluents for refrigerated storage.
  • The sulphanilamide is not added but the glycerol is added at the level of 10%.

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Lactose egg yolk extender

  • The sugars like glucose, lactose, raffinose and sucrose have been used with semen for freezing bull spermatozoa.
  • The results indicated that sperm survival rate increased as the molecular weight of the sugar is increased.

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Last modified: Monday, 4 June 2012, 11:31 AM