Veterinary Andrology and Reproductive Techniques

COMPOSITION OF TRIS EGG YOLK GLYCEROL/UNIVERSAL DILUENT

For preparing 1 litre diluent

S.No.	Contents	Quantity
1	Tris	30.28 gm
2	Citric acid	16.75 gm
3	Fructose	12.50 gm
4	Glycerol	70 ml
5	Penicillin G sodium	10 lac unit
6	Dihydrostreptomycin	1000 mg
7	Distilled water (make up to)	800 ml
8	Egg yolk (finally added)	200 ml

Glycerol and egg yolk are added @ 7% and 20% respectively.

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