Principles of Biochemistry (2+1)

Practical

Preparation of chemical solutions, buffers and reagents.Estimation of glucose and protein in fish tissues.  Extraction and estimation of total lipids from fish tissue. Applications of paper chromatography, thin layer chromatography, ion exchangechromatography. Principles of electrophoresis. Estimation and separation of proteins by electrophoresis; Determination of saponification and iodine values. Determination of lipase activity. Gel filtration. Preparation of tissue homogenate and organelle isolation.