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4.2.3.2 Reaction with Sanger’s reagent
A very important reaction of the amino groups of amino acids is with 1- fluoro -2,3 - dinitrobenzene, FDNB, also called “Sanger’s reagent”. The reagent condenses with free amino groups in the cold in mildly alkaline solution (bicarbonate) to form dinitrophenyl(DNP) amino acid which is a fluorescing compound which is used for analysis of amino acids by chromatography.
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Last modified: Thursday, 10 November 2011, 10:29 AM