Principles of Biochemistry (2+1)

5.3.2.2.2.4 Affinity chromatography

Affinity chromatography is a very useful technique for "polishing" or completing the protein purification process. Beads in the chromatography column are cross-linked to ligands that bind specifically to the target protein. The protein is then removed from the column by rinsing with a solution containing free ligands. This method generally gives the purest results and highest specific activity compared to other techniques.