Principles of Biochemistry (2+1)

Reagents

1. Lipase substrate: Olive oil, 50% (v/v) and sodium azide, 0.1% added as preservative. Shake vigorously for a minimum of 20 sec. before using. Store at room temperature (18- 26ºC). Do not freeze.
2. Trizma buffer (Tris (hydroxymethyl) amino methane) 0.2 mol. pH 8.0 at 25ºC: Add 0.1% sodium azide as preservative. Store at room temperature (18-26ºC). Discard if turbidity develops.
3. Thymolphthalein indicator: Thymolphthalein 0.9% (W/V) in ethanol. Store tightly capped at room temperature (18-26ºC).
4. Ethanol : 95%
5. Sodium hydroxide 0.05 N: Prepare 0.1NaOH solution and standardize by titration against a standard 0.1 acid. Using the formula V₁ N₁ =V₂ N₂ find out the normality.  

Then prepare exactly 0.05 N NaOH solution. Store tightly capped in pyrex or polyethylene bottle.