Principles of Biochemistry (2+1)

Reagents

1.  Mobile phase (solvent system)

• Mix n-butanol, glacial acetic acid and water in the ratio of 4:1:5 in a separating funnel and let it stand to equilibrate for 30 min. 
• Drain off the lower aqueous phase into a beaker and place it inside to saturate the chromatography chamber. 
• Save the upper organic phase and use it for developing the chromatogram

2. Standard amino acids

• Dissolve different individual amino acids in distilled water at a    concentration of 1 mg/ml.
• Use very dilute (0.05 N) HCl to dissolve the free amino acids tyrosine and phenylalanine. 
• Dissolve tryptophan in a very dilute (0.05 N) NaOH solution

3. Ninhydrin reagent

• Dissolve 100 mg of Ninhydrin in 100 ml acetone.