Taxonomy of Finfish (1+2)

6.1.5.5 C-Banding

C- band can be demonstrated by

1. BSG (barium hydroxide/saline/Giemsa) technique developed by Sumner (1972) is regarded as the standard C-banding method. Slides are hydrolysed by 0.2N HCl, denatured in barium hydroxide solution, treated with 2×SSC (saline sodium citrate) and stained with Giemsa.
2. By staining the metaphase chromosome with restriction enzymes, C- band can also be demonstrated.

Information on C-banding is available in over 100 species of fishes. C-banding in fishes includes not only the centromeric region but often it demonstrates heterochromatin at the telomeric and inter-calary region. In some cases the whole arm is found to be C-band positive.