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3.3.2. Preservation of pituitary gland
Unit 3 - Breeding of major carps
3.3.2. Preservation of pituitary gland
1. Preservation in absolute alcohol
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In this method, the gland, after collection, is immediately transferred to a vial/phial containing fresh absolute alcohol (ethanol).
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After 24 hours, the alcohol is removed and fresh alcohol is added and stored at room temperature or in a refrigerator.
2. Preservation in acetone
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Immediately after collection, the pituitary gland is kept in ice-chilled acetone and stored in a refrigerator for 2-3 days.
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After this period, the acetone is changed and the gland stored in a refrigerator.
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Both absolute alcohol and acetone have de-fattening and dehydrating effect.
3. Immediate freezing
- In this method, the collected glands are frozen immediately and stored in a freezer.
Last modified: Thursday, 9 June 2011, 9:31 AM