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Determination of fatty acids of fish by gas chromatography
Lipids present in the fish muscle are extracted using chloroform. The extracted lipids are esterified using boron trifluoride (BF3)-methanol and converted to methyl esters. The fatty acid methyl esters are then separated individually in the gas chromatograph based on the number of carbon chains and boiling temperature. |
Last modified: Saturday, 12 November 2011, 7:23 AM