Biochemical Techniques and Instrumentation (1+2)

Reagents

1. Blotting transfer buffer (6L)
   20mM Tris base - 14.52g/6l
   150mM glycine - 67.56g/6l
   20% methanol - 1200ml/6l
2. Ponceau S
3. Buffer A (300 ml)
   10mM Tris, pH 7.5 - 3.0ml of 1M Tris pH 7.5
   0.15M NaCl - 11.25ml of 4M NaCl
   0.5% BSA - 1.5g BSA
   1% gelatin - 3.0g gelatin
   Heat to ~50^oC to dissolve
4. Buffer B
   Buffer A + 0.1% Tween 20
   (0.1ml of Tween 20 per 100ml of Buffer A)
5. Buffer C (1L)
   10 mM Tris pH 7.5 - 10ml of Tris pH 7.5
   0.15M NaCl - 37.5ml of 4M NaCl
   0.1% Tween 20 - 1ml of Tween 20
6. Primary antibody
7. Radioactive label 125 I-Protein A