Site pages
Current course
Participants
General
Topic 1
Topic 2
Topic 3
Topic 4
Topic 5
Topic 6
Topic 7
Topic 8
Topic 9
Topic 10
Topic 11
Topic 12
Topic 13
Topic 14
Topic 15
Topic 16
Topic 17
Topic 18
Topic 19
Topic 20
Topic 21
Topic 22
Topic 23
Topic 24
Topic 25
Topic 26
Materials
1. Bicarbonate/carbonate coating buffer (100 mM) Antigen or antibody should be diluted in coating buffer to immobilize them to the wells: Na2CO3 - 3.03 g NaHCO3 - 6.0 g Distilled water - 1000 ml pH 9.6, 2. Phosphate buffered saline (PBS) Na2HPO4 - 1.16 g KCl - 0.1 g K3PO4 - 0.1 g NaCl - 4.0 g Distilled water - 500 ml pH 7.4. 3. Blocking solution Commonly used blocking agents are 1% BSA or 5% non-fat dry milk in PBS. 4. Wash solution Usually PBS or Tris -buffered saline (pH 7.4) with detergent such as 0.05% (v/v) Tween20. 5. Antibody dilution buffer Primary and secondary antibody should be diluted in 1X blocking solution to reduce non specific binding. 6. Substrate 7. Enzyme conjugale 8. Stop solution |
Last modified: Saturday, 12 November 2011, 7:52 AM