Culture of fish food organisms (1+1)

Unit 8 - Brine Shrimp or Artemia

8.5.2. Reaction with decapsulation solution (hypochlorite)
Prepare the decapsulation solution using 1N NaOH, sodium hypochlorite (NaOCI) and seawater.
Allow the hydrated cysts to react with the decapsulation solution (hypochlorite) for 7–15 minutes. To prevent damage of embryo, keep the temperature below 40°C by adding ice cubes to the suspension or by using a water bath. A change in colour of the cysts from brown to white to orange usually indicates that the reaction is complete. Check under the microscope if possible.