Biotechnology and Bioinformatics (1+1)

Immunodot

ImmunoDOT utilizes an enzyme-linked immunoassay (EIA) dot technique for the detection of antibodies/ antigens. The suspected sample/ test antigen is dispensed as discrete dots onto a solid membrane. After adding specimen to a reaction vessel, an assay strip is inserted, allowing test antibodies (in the case of antibody detection) or primary antibody (in case of antigen detection) reactive with the test antigen/ sample to bind to the strip's solid support membrane. In the second stage, the reaction is enhanced by removal of non-specifically bound materials. During the third stage, an enzyme conjugated secondary antibody is allowed to react. Finally, the strip is transferred to a substrate reagent, which reacts to produce an easily seen, distinct dot.