Biotechnology and Bioinformatics (1+1)

Materials required

· Nitrocellulose (NC) or polyvinylidene fluoride ( PVDF ) membrane

· Suspected sample or test antigen

· Test antibody

· Purified primary antibody ( IgG )

· Secondary antibody conjugated to enzyme (HRP or alkaline phosphatase)

· Substrate solution (DAB: 3,3´-diaminobenzidine tetrahydrochloride or NBT-BCIP: 5-bromo-4-chloro-3-indolyl-phosphate/ nitro-blue tetrazolium)

· Phosphate buffered saline (PBS)

· PBST (PBS with 0.5% Tween 20)

· Blocking solution (0.5% skim milk powder solution in PBS)

· Hydrogen peroxide