VPB 112: General Veterinary Biochemistry (1+1)

Procedure for checking the buffering capacity of various acetate buffers

PROCEDURE

• 5 ml of 0.1 N HCl is to be added to 90 ml of various concentrations of the above acetate buffers. Then determine their pH using the pH meter as mentioned above.

Observation

S.No.	T.T. No.	Volume of 0.1N CH3COONa	Volume of 0.1 N CH3COOH	Total Volume	Initial pH	Vol. of 0.1N HCl	pH (after addition of 0.1N HCl
1	I	10 ml	80 ml	90 ml		5 ml
2	II	30 ml	60 ml	90 ml		5 ml
3	III	50 ml	40 ml	90 ml		5 ml
4	IV	70 ml	20 ml	90 ml		5 ml
5	V	90 ml	-----	90 ml		5 ml
6	VI	-----	-----	90 ml D.W		5 ml

Result

• The pH values of the various acetate buffers prepared are shown in the observation table.
• Buffering Capacity: