VPB 112: General Veterinary Biochemistry (1+1)

TITRATION CURVE - PROCEDURE AND RESULT

Procedure

Titration of acetic acid vs. sodium hydroxide

• Prepare 100 mL of each 0.1N CH₃COOH and O.1N NaOH.
• Take 50 mL of 0.1N CH₃COOH in a beaker.
• Measure the initial pH of the 0.1N CH₃COOH, using a pH meter.
• Add exactly 2 mL of 0.1N NaOH from the burette, mix well and measure the pH.
• Repeat the above procedure till the pH of the solution is about 8 –10.
• Plot a graph taking the volume of NaOH added in X-axis and pH values in Y- axis.
• Connect all the points and observe the nature of this titration curve.
• Find out the pKa of acetic acid, exactly at the half-saturation point of the titration.

Result

• The pKa value of acetic acid is _________.