VPB 112: General Veterinary Biochemistry (1+1)

TITRATION CURVE - PRINCIPLE

• Titration is a procedure in which we measure the quantity of a known reagent required to react with an unknown sample, from which we deduce the concentration of the unknown.
• The NaOH is added in small increments to a known volume of weak acid till neutralization, as determined by the pH meter.
• The plot of pH against the volume of NaOH added is called as the ‘Titration Curve”. This curve reveals the pKa (negative logarithm of dissociation constant) of an acid.