VPB 121: Veterinary Physiology - II (2+1)

PREPARATION

Preparation of the instrument for collection of rumen fluid

• The suction strainer is unscrewed and the perforations at the end of the nylon tube should be clean. The full length of the nylon perforated end is projected out.
• The suction pump for its working condition is checked.
• The two ways ‘T’ joint is fixed tightly to the sampling bottle to prevent the air leakage.
• The nylon tube from the suction strainer is fixed into the vertical end of the two ways ‘T’ joint. Horizontal end of joint should be connected to the suction pump.
• The entire set is checked by dipping the suction strainer into a bucket of water and operating the suction pump.
• Liquid paraffin is applied over the stainless steel spiral sound of the instrument which is ready for the collection.

Important points to be observed before and after the collection of rumen fluid

• About 200ml of rumen fluid sample is to be collected for various tests.
• The rumen fluid can be kept in room temperature for five hours and in refrigerator for 24 hours
• After collection, rumen fluid container should be immediately closed air tightly
• The first collection of rumen fluid is discarded, so that error in pH can be minimized