VPB 121: Veterinary Physiology - II (2+1)

RECORDING OF INTESTINAL MOTILITY - PROCEDURE

• Prepare the organ bath to maintain the temperature at 38^oC, fill the container with Tyrode solution.
• Anaesthetise the rabbit by injecting Ketamine @ 25mg/kg body weight i/m in combination with Midazolam @ 5mg/kg body weight intra muscularly.
• Shave the abdominal region and clean the area with warm water.
• After cleaning, the skin can be sterilized by swabbing with 70% ethanol.
• Open the abdominal cavity by making linear incision along the linea alba.
• Remove gently a few 3-4cm long portions of jejunum (without mesentery) and oxygenate them frequently.
• Using a needle put a thread onto the wall of one of the isolated intestine place from inside to outside and make a small loop, leaving the thread in place.
• Pass a threaded needle to the other end of the intestinal piece, tie a knot and leave about 15cm thread.
• The long free end of this thread is connected to the lever.
• Do not close the lumen of the gut.
• Pass the loop over the O₂ tube and tie the intestine with it and put this tube into the inner vessel and fix it in position.
• Connect the oxygen tube to O₂ cylinder and allow 2-3 bubbles of O₂ to pass through Tyrode solution per minute.
• Carefully adjust the lever to mark the kymograph. Record the intestinal movements on the kymograph.
• Add 1 ml of adrenaline to the Tyrode solution and record its effect.
• Completely drain the Tyrode solution, replace fresh Tyrode solution, record the normal in­testinal motility.
• Add 0.5 ml of acetylcholine to Tyrode solution and record its effect.