VMC 221: Veterinary Immunology and Serology (1+1)

MATERIALS 

• Saturated ammonium sulphate solution
  • Ammonium sulphate
  • Distilled water
  • The salt was dissolved at 50 ⁰ C and was allowed to stand at room temperature overnight.
  • The pH was adjusted to 7.2 with dilute ammonia or sulphuric acid.
• Latex beads – Latex beads (0.8 um) was used.
• Carbonate bicarbonate buffer:
  • Sodium carbonate - 1.5 g
  • Sodium bicarbonate - 2.93 g
  • Distilled water - 1000 ml
  • The pH of the solution was adjusted at 9.6 before making up to 1000 ml.