VPT 311: General and Systemic Veterinary Pharmacology (2+1)

INVITRO ANTICOAGULANTS

• Anticoagulants useful for blood collection for laboratory use include
  • Sodium oxalate 20% solution at 0.01ml/ml of blood
  • Sodium citrate 25% solution at 0.01ml/ml of blood
  • Edetate disodium (EDTA) 2% solution at 0.01ml/ml of blood
  • Heparin sodium 75 IU/10 ml of blood
• Anticoagulants useful for blood collection for transfusion include
  • Sodium citrate solution at 10 ml/90 ml of blood containing Sodium citrate 2.5 g, sodium chloride 0.9 g and distilled water to make 100 ml
  • ACD solution – Acid citrate dextrose solution at 15 ml/100 ml of blood containing Sodium citrate 2.5 g, citric acid 0.8 g, dextrose 2.5 g and distilled water to make 100 ml
  • Heparin sodium at 400 to 600 IU/100 ml of whole blood
• In addition to the above agents applying cold at 2 to 5 C or collecting blood in a receptacle having smooth or unwettable walls like silicon coated receptacles can also be used to collect blood without coagulation.