VPP 322: Aquatic Animal Diseases, Health Care and Management (1+1)

DIAGNOSIS OF FISH DISEASES

• Sample size required to detect one or more infected specimens in populations (lots) with an assumed minimum prevalence of detectable infection of 5 and 10%. Calculations are based on a 95% level of confidence. For intermediate population sizes, use the sample size for the next larger population listed.

Number of fish to be sampled when assumed prevalence of detectable infection

Population Size	5%	10%
50 100 250 500 1,000 2,500 5,000 10,000 100,000 Over 100,000	29 43 49 54 55 56 57 57 57 60	20 23 25 26 27 27 27 27 27 30

• Fish samples must be handled rapidly in such a way that degenerative changes do not render diagnosis unreliable or impossible. If samples cannot be brought to the laboratory alive, they should be stored on ice or refrigerated for no longer than 48 hours.

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