VPB 321: Animal Biotechnology (2+1)

MODERN VACCINES

• Two main approaches are being used to develop vaccines using recombinant DNA technology.
  • The first involves deleting genes that determine the virulence of the pathogen, thus producing attenuated organisms (non-pathogens) that can be used as live vaccines. Currently, this strategy is more effective against viral and bacterial diseases than against parasites. Attenuated live vaccines have been developed against the herpes viruses that cause pseudorabies in pigs and infectious bovine rhinotracheitis in cattle. A number of candidate Salmonella vaccines have also been produced.
  • The second approach is to identify protein subunits of pathogens that can stimulate immunity. The International Livestock Research Institute (ILRI) used this approach to develop a vaccine against Theileria parva, the parasite that causes East Coast fever in African cattle.
• A recent approach has been to use vaccines based on DNA. The use of DNA in vaccines is based on the discovery that injecting genes in the form of plasmid DNA can stimulate an immune response to the respective gene products. This immune response is a result of the genes being taken up and expressed by cells in the animal after injection. The live-vector and DNA vaccination systems could be manipulated further to enhance the immunity conferred by the gene products.