VPB 321: Animal Biotechnology (2+1)

TRANSFECTION

• Transfection is the process of introducing nucleic acids into eukaryotic cells by using carrier molecules.
• Animal cells are transfected by opening transient pores or holes in the cell membrane to allow the uptake of material.
• Genetic material such as plasmid DNA or siRNA constructs or proteins such as antibodies may be transfected into cells.
• Transfection may cause changes in morphology of target cells.