VPB 321: Animal Biotechnology (2+1)

PHYSICAL MAPPING

• In physical mapping, the DNA is cut by a restriction enzyme.
• Once cut, the DNA fragments are separated by electrophoresis.
• The resulting pattern of DNA migration (i.e., its genetic fingerprint) is used to identify what stretch of DNA is in the clone.
• By analysing the fingerprints, contigs are assembled by automated finger printed contigs (FPC) or manual means (Pathfinders) into overlapping DNA stretches.
• Macrorestriction is a type of physical mapping wherein the high molecular weight DNA is digested with a restriction enzyme having a low number of restriction sites.
• Once the map is determined, the clones can be used as a resource to efficiently contain large stretches of the genome. This type of mapping is more accurate than genetic maps.