VPT 321: Veterinary Neuropharmacology (2+1) copy 1

COMPOSITION OF SOME COMMONLY USED PHYSIOLOGICAL SALT SOLUTIONS

	Quantities for 10 litres of
	Frog-Ringer	Krebs	Tyrode	Ringer-Locke	De Jalon
NaCl	65 g	69 g	80 g	90 g	90 g
KCl 10 Per cent	14 ml	35 ml	20 ml	42 ml	42 ml
MgSO4, 7H2O 10 per cent	-	29 ml	26 ml	-	-
NaH2PO4, 2H2O 5 per cent	1.3 ml	-	13 ml	-	-
KH2PO4 10 per cent	-	16 ml	-	-	-
Glucose	20 g	20 g	10 g	10 g	5 g
NaHCO3	4 g	21 g	10 g	5 g	5 g
CaCl2 (Molar)	10.8 ml	25.2 ml	18 ml	10.8 ml	2.7 ml
Aerating gas	Air	O2 + 5% CO2	O2 or air	Pure O2	O2 + 5% CO2

Note

• For amphibian tissue use frog –Ringer solution
• For mammalian or avian skeletal muscle use Kreb’s solution
• For intestine use Tyrode’s solution
• For heart muscle use Ringer-Locke solution
• For the rat uterus preparation use De Jalon’s solution
• Mammalian tissues sometimes require aeration with O₂ + 5% CO₂