VLD 411: Veterinary Clinical Biochemistry and Laboratory Diagnosis - I (0+1)

PRINCIPLE

Direct (or) Conjugated Bilirubin

• It diazotizes with sulfanilic acid to form azobilirubin, a purple coloured complex in acid medium.

Indirect (or) Unconjugated Bilirubin

• It is diazotized only in presence of methanol to give a purple coloured complex
• Both direct and indirect reaction represents the total bilirubin concentration.
• The difference of the total and direct gives the indirect bilirubin.