VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

CALCULATION

• A standard curve is prepared by taking 0.02 – 0.1% standard fructose solution in saturated benzoic acid.
• The amount of fructose is calculated from this curve.
• Blank is prepared by adding all the reagents and in place of semen water is added.
• The amount of fructose present in semen immediately after collection and present after one hour’s incubation are worked out.
• The difference between the two concentrations of fructose is on account of fructolysis in one hour.
• Fructolysis may also be worked out in respect of 10⁹ live spermatozoa.
• This method is useful as compared to 10⁹ sperm concentration and provides a true comparison of metabolic activities of semen samples.

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