Resistance to environment

RESISTANCE TO ENVIRONMENT

High temperature viability test

There is high correlation between the time of incubation at 1000F required for all spermatozoa to loose their motility and conception rate.

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Cold shock resistant test

  • This test is done to assess the fertilizability/preservability (5⁰C) of the semen of a particular bull.
  • The spermatozoa are subjected to unfavourable condition such as cold (0⁰C).
  • The percentage of resistant live sperm is counted after cold shock.
  • There is a good correlation between percentage of sperms which withstand cold shock and motility after 5 days of preservation under chilled condition (5⁰C).

Materials required

  • Test tube
  • Semen sample
  • Microscope
  • Crushed ice
  • Eosin and Nigrosin stain

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Procedure

  • Examine the live sperm percentage of the fresh semen immediately after collection.
  • Take one ml of freshly collected in a test tube and place it in a beaker containing crushed ice (0⁰C) for 10 minutes.
  • Determine the percentage of live sperm and motility after cold shock.
  • Compare the percentage of live sperm in fresh semen sample and semen after expose to crushed ice.
  • Then estimate the storage ability and freezability of spermatozoa.

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Resistance to sodium chloride

The amount of 1% sodium chloride solution required to cease the motility refelects the resistance of spermatozoa.

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Last modified: Monday, 4 June 2012, 11:35 AM