VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

DILUTION OF SEMEN

• Immediately after semen collection the volume, concentration and initial motility are estimated.
• A minimum of 2.5 ml volume, 500 million concentration and 70% initial motility is required to process a semen sample.

The minimum requirements to accept a semen sample is

• Volume - 2.5 ml
• Concentration - 500 millions
• Initial motility - 70%

• The semen is diluted initially with 1:1 dilution for assessing the initial motility.
• Then the dilution rate is calculated as per the above method (depends upon the packing volume) and the final dilution is made in a sterile beaker.
• While diluting the semen the diluents should not be poured directly over the semen.
• It should be poured in the side walls of the beaker without air bubble formation.