Qualitative estimation of cynide in biological material
QUALITATIVE ESTIMATION OF CYANIDE IN BIOLOGICAL MATERIAL
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Aim
Principle
Materials
- Tartaric acid
- copper sulfate
- Alcohol
- Guaiacol
- Filter paper
- 100 ml flask and
- Heater
Procedure
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Take 50 g of finely ground tissue or stomach contents in a 100 ml flask and acidify the contents with tartaric acid
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Take a filter paper previously moistened with 10 % guaiacol in alcohol and 0.1% of aqueous copper sulfate solution
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Hang the filter paper in the flask so that it does not touch the contents in the flask, plug the mouth of flask and warm gently
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Allow it to stand for 30 minutes
- Observe the colour of the paper
Inference
- If cyanide is present, blue colour develops
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Last modified: Wednesday, 16 May 2012, 9:17 AM