Principles of Plant Breeding (2+1)

Analysis of marker segregation

Analysis of marker segregation in the mapping population

• The individual plants of the mapping population are analyzed using the same primers which detect polymorphism between parents.
• The plants are categorized as parent 1 type, parent 2 type or F1 depending on the size of the polymorphic fragments.
  
• The observe frequency of the plants based on the marker locus genotypes is compared with the expected segregation ratio of 1:2:1 in case of F2 and 1:1 in case other populations. Based on the chi-square analysis, the observed segregation pattern is tested for possible distortion.