Analysis of marker segregation


Analysis of marker segregation

    Analysis of marker segregation in the mapping population
    • The individual plants of the mapping population are analyzed using the same primers which detect polymorphism between parents.
    • The plants are categorized as parent 1 type, parent 2 type or F1 depending on the size of the polymorphic fragments.
    • The observe frequency of the plants based on the marker locus genotypes is compared with the expected segregation ratio of 1:2:1 in case of F2 and 1:1 in case other populations. Based on the chi-square analysis, the observed segregation pattern is tested for possible distortion.

Last modified: Monday, 12 March 2012, 7:07 AM