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Generation of a suitable mapping population
Selected plants are crossed to obtained f1 hybrids from which four different mapping population can be generated: F2 (by Selfing a single F1 plant ) , BC (by crossing F1 with either of parents) ,Recombinant Inbredlines (by Selfing individual F2 plants for 7-8 generations), and doubled haploids (by invitro culture of anthers or pollens from the F1 followed by chromosomes doubling).50-100 F2 plants are required for construction of linkage map.
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Last modified: Monday, 12 March 2012, 7:08 AM