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5.3.2 Principles of Protein Purification
Proteins must be modified with optimized properties for specific industrial applications. In order to do this proteins of interest must be isolated and purified so that their conformations, substrate specificities, reactions with other ligands, and specific activities can be studied. The degree of protein purity required depends on the intended end use of the protein. For some applications, a crude extract is sufficient. However, for other uses, such as in foods and pharmaceuticals, a high level of purity is required. In order to achieve this, several protein purification methods are typically used, in a series of purification steps. |
Last modified: Monday, 27 February 2012, 6:32 AM