Biochemical Techniques and Instrumentation

 Theory

Unit I. Spectrophotometry

Chapter 1. Theory and applications of Spectrophotometry

Chapter 2. UV-Vis Spectrophotometry and its instrumentation 

Unit II. Chromatography  

Chapter 1. Basic principles of chromatographic techniques

Chapter 2. Thin layer chromatography

Chapter 3. Gel filtration

Chapter 4. Ion Exchange Chromatography

Chapter 5. Affinity Chromatography

Chapter 6. Gas Chromatography

Chapter 7. High Pressure Liquid Chromatography  

Unit III. Immunological Techniques

Chapter 1. Radio isotopes and radio Immuno assay
Chapter 2. ELISA

Unit IV. Centrifugation.

Chapter 1. Principles of centrifugation
Chapter 2. Ultracentrifugation

Unit V. Blotting techniques

Chapter 1. Southern Blotting

Chapter 2. Northern Blotting

Chapter 3. Western Blotting

Unit VI. Molecular techniques

Chapter 1. Polymerase chain reaction

Chapter 2. Cloning
Chapter 3. Cell culture
Chapter 4: Hybridoma technology