Biochemical Techniques and Instrumentation

Materials and Procedure

Materials

1. Tris - EDTA (TE) buffer

Procedure

1. Isolation of DNA from tissue/cell
2. Take 1ml TE (Tris - EDTA) buffer in a cuvette and calibrate the spectrophotometer at 260nm as well as 280nm.
3. Add 10μl of each DNA sample to 900 μl TE buffer and mix well.
4. Use TE buffer as a blank in the other cuvette of the spectrophotometer.
5. Note the OD₂₆₀ and OD₂₈₀ values on spectrophotometer.
6. Calculate the OD₂₆₀/OD₂₈₀ ratio.