Biochemical Techniques and Instrumentation

Comments

• A ratio between 1.8-2.0 denotes that the absorption in the UV range is due to nucleic acids.
• A ratio lower than 1.8 indicates the presence of proteins and/or other UV absorbers.
• A ratio higher than 2.0 indicates that the samples may be contaminated with chloroform or phenol. In either case (<1.8 or >2.0) it is advisable to re-precipitate the DNA.