Biochemical Techniques and Instrumentation

Determination of fatty acids of fish by gas chromatography

Lipids present in the fish muscle are extracted using chloroform. The extracted lipids are esterified using boron trifluoride (BF₃)-methanol and converted to methyl esters. The fatty acid methyl esters are then separated individually in the gas chromatograph based on the number of carbon chains and boiling temperature.