Biochemical Techniques and Instrumentation

Materials

1. Gas chromatograph
2. Rotary flask evaporator
3. Distillation apparatus
4. Separating funnels
5. Reflux condensors
6. Solvents : Chloroform, methanol, hexane, diethyl ether
7. 0.74% Potassium chloride (KCl) : Dissolve 0.74 g of KCl in 100 ml of distilled water
8. 1% Sulfuric acid in methanol : Dissolve 1 ml of concentrated sulfuric acid in 100 ml of methanol
9. 5% Sodium chloride (NaCl): Dissolve 5 g NaCl in 100 ml of distilled water
10. 2% Potassium bicarbonate : Dissolve 2 g of potassium bicarbonate in 100 ml of distilled water
11. Sodium sulfite, anhydrous
12. 0.05N Alcoholic NaOH: Dissolve
13. BF₃ methanol
14. Saturated sodium chloride (25%) : Dissolve 25 g of NaCl in 100 ml of distilled water
15. Sigma fatty acid methyl ester (FAME) mixture (C4 – C24)