Biochemical Techniques and Instrumentation

Stripping Southern Blot on Nitrocellulose

Treat blot with:

1. 0.5 N NaOH x 10 min. at R.T. (250 ml)
2. 0.5 N NaOH x 10 min. at R.T.
3. 1 M Tris pH 7.6 x 10 min. at R.T. x 2

Dry blot slightly on Whatman, immediately place into hybridization cocktail.