Reagents
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20xSSC Sodium chloride 175.3g
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Sodium citrate 88.2g
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Distilled water 800ml
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Adjust to pH 7.0
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Make up to 1l with distilled water.
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Dilute 1:1000 and check conductivity (around 450mS)
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Autoclave and store at room temperature for a maximum of 12 months.
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Records: Record batch numbers of 20xSSC and Hybond N on the gel blot Logsheet.
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Last modified: Saturday, 12 November 2011, 7:37 AM