Biochemical Techniques and Instrumentation

Materials

1. PCR primer (invA)
2. dNTPs (100mM each)
3. Taq DNA polymerase (3 U/ml)
4. PCR buffer with MgCl₂
5. Ethidium bromide (5 mg/μl)
6. Agarose (2%) in 1X TAE
7. 6 X Gel loading dye
8. 1 X TAE buffer (pH 8.5)
   Dissolve 48.4g Tris base and 7.44 g Di-sodium EDTA in 1.42 ml glacial acetic acid and make upto 1000 ml with molecular grade water