Biochemical Techniques and Instrumentation

Reagents

1. Alkaline Solution I
50 mM glucose
25 mM Tris HCl (pH 8.0)
10 mM EDTA (pH 8.0)

2. Lysozyme, 10 mg/ml

3. Alkaline Solution II
0.2 N NaOH (2.0 ml of 10 N NaOH in 100ml double distilled water)
1.0% SDS

4. Alkaline Solution III
5 M Potassium acetate
Glacial acetic acid
Autoclaved water

5. TE buffer
10 mM Tris HCl (pH 8.0)
1.0 mM EDTA (pH 8.0)

6. Ampicillin 100 µg/ml

7. Isopropyl alcohol, Ice cold

8. Luria Broth media
Tryptone - 1 gm
Yeast extract - 0.5 gm
Sodium chloride - 1 gm
Deionised water - 75 ml
Adjust the pH to 7.0 with 5 N NaOH and then make up the volume to 100 ml with deionised water. Sterilize it by autoclaving it for 20 minutes at 15 psi.